Journal
JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
Volume 84, Issue -, Pages 102-107Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.molcatb.2012.02.001
Keywords
One-pot two-step oxidation/aldol reaction; Biocatalytic oxidation; Laccase/O-2/TEMPO; Alcohol oxidase; D-Fructose-6-phosphate aldolase
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In situ coupling of aldehyde generation, by a mild alcohol oxidation, with an enzymatic aldol addition reaction, mediated by D-fructose-6-phosphate aldolase (FSA) has been investigated as an approach to improve the performance of the process. Four sustainable oxidation methods compatible with the activity and stability of the enzymatic aldol addition have been assayed. Among them, the laccase/O-2/2,2,6,6-tetramethylpiperidine-N-oxyl (TEMPO) and alcohol oxidase gave the best results for the N-Cbz-aminoethanol to N-Cbz-glycinal (53%) and furfuryl alcohol to furfural (89%), respectively, followed by the aldol addition with hydroxyacetone catalyzed by FSA A129S mutant. (C) 2012 Elsevier B.V. All rights reserved.
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