Journal
JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
Volume 68, Issue 2, Pages 206-210Publisher
ELSEVIER
DOI: 10.1016/j.molcatb.2010.11.005
Keywords
Direct electrochemistry; Carbon nanotube; TiO2; Glucose oxidase
Funding
- Research Councils of Sharif University of Technology
- University of Tehran
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A nano-composite material consisting of amine functionalized TiO2-coated carbon nanotubes was prepared and used for glucose oxidase (GOx) absorption. The GOx bearing nanomaterial was fixed on a glassy carbon electrode to construct a novel biosensor for glucose determination. The direct electrochemistry of immobilized GOx and its electron transfer parameters at the modified glassy carbon electrode were reported. The apparent heterogeneous electron transfer rate constant (k(s)) of GOx was estimated to be 3.5 s(-1), which is higher than those reported previously. Amperometric detection of glucose resulted in a rapid (3 s) and stable response in the linear concentration range from 1.8 to 266 mu M. The apparent Michaelis-Menten constant was measured to be 8.59 mM, indicating that the immobilized GOx on NH2-TiO2-CNT matrix retained its native activity. The anti-interference ability and long-term stability of the biosensor were also assessed. (c) 2010 Elsevier B.V. All rights reserved.
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