Journal
JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
Volume 68, Issue 1, Pages 66-70Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.molcatb.2010.09.013
Keywords
Ionic-liquid buffer; Hydroxyl-functionalized ionic liquid; Lipase; Fluorescence
Funding
- NSFC [20873108, 20923004]
- Science Foundation of Jimei University [2009CB939804]
- Key Scientific Project of Fujian Province [2009HZ0002-1]
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Ionic-liquid buffer having phosphate anion was synthesized for the development of buffered enzymatic ionic liquid systems Both the conformation and transesterification activity of Candida antarctica lipase B (CALB) dissolved in the hydroxyl-functionalized ionic liquids were buffer dependent Intrinsic fluorescence studies indicated that the CALB possessed a more compact conformation in the medium consisted of ionic liquid buffer having phosphate anion and hydroxyl-functionalized ionic liquids like 1-(1-hydroxyethyl)-3-methyl-imidazolium tetrafluoroborate and 1-(1-hydroxyethyl)-3-methyl-imidazolium nitrate High activity and outstanding stability could be obtained with the CALB enzyme in the buffered ionic liquids for the transesterification (c) 2010 Elsevier B V All rights reserved
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