Journal
JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
Volume 68, Issue 3-4, Pages 250-255Publisher
ELSEVIER
DOI: 10.1016/j.molcatb.2010.11.013
Keywords
Xylosidase; Biotransformation; 7-Xylosyl-10-deacetyltaxol; 10-Deacetyltaxol; Enterobacter sp.
Funding
- Program for New Century Excellent Talents in University [NCET-06-0155]
- Guilin Hui'ang Biochemistry Medicine Industry Co. Ltd., China
- National Science & Technology Major Project 'Key New Drug Creation and Manufacturing', China [2009ZX09301-003-4-1]
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Enterobacter sp. CGMCC 2487, a bacterial strain isolated from the soil around a Taxus cuspidata Sieb. et Zucc. plant, was able to remove the xylosyl group from 7-xylosyltaxanes. The xylosidase of this strain was an inducible enzyme. In the bioconversion of 7-xylosyl-10-deacetyltaxol (7-XDT) to 10-deacetyltaxol (10-DT), for the purpose of enhancing the conversion efficiency, the effects of NH4+, oat xylan, temperature, pH value, cell density and substrate concentration on the bioconversion have been systematically investigated. 3.0 mM NH4+, 0.6% oat xylan in the media could enhance the yield of 10-DT; the optimum biocatalytic temperature was 26 degrees C and optimum pH value was 6.0. The highest conversion rate and yield of 10-DT from 7-XDT reached 92% and 764 mg/L, respectively. In addition, the biocatalytic capacity of the cell cultures remained 66.1% after continuous three batches. These results indicate that converting 7-XDT to 10-DT, a useful intermediate for the semisynthesis of paclitaxel or other taxane-based anticancer drugs by a novel bacterial strain, Enterobacter sp. CGMCC 2487, would be an alternative for the practical application in the future. (C) 2010 Elsevier B.V. All rights reserved.
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