Journal
JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
Volume 71, Issue 3-4, Pages 90-94Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.molcatb.2011.04.003
Keywords
Endophytic microorganisms; beta-Keto esters; Bioreduction; Biocatalysis
Funding
- ANII [FCE2007_562]
- PEDECIBA Quimica
- OPCW
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Several strains of endophytic bacteria and yeast were isolated from Daucus carota, Erythrina crista-galli, Curcubita maxima and Fortunella margarita, and tested for the reduction of two model alpha-alkyl-beta-ketoesters. The yeast strains resulted better biocatalysts for the reduction of the tested substrates with the exception of Enterobacter agglomerans C8 strain, isolated from Erythrina crista-galli, that provided the best results for the preparation of syn (2R,3S)alpha-alkyl beta-hydroxyesters. Among the isolated yeast strains, the Pichia sp. strain isolated from D. carota, was the best biocatalyst for production of anti (2S,3S)alpha-alkyl beta-hydroxyesters. Our results show that the microbial endophytic community provides an interesting niche on the search for novel biocatalysts. (C) 2011 Elsevier B.V. All rights reserved.
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