Journal
JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
Volume 58, Issue 1-4, Pages 103-109Publisher
ELSEVIER
DOI: 10.1016/j.molcatb.2008.12.001
Keywords
Esterification; Ionic liquids; Lipase immobilization; Magnetic nanoparticle
Funding
- National Natural Science Foundation of China [20876158, 20676137]
- National High Technology Research and Development Program of China [2006AA02Z215]
- Chinese Academy of Sciences [KSCX2-YW-G-019]
- Advanced Research Project of Institute of Processing Engineering, CAS [2007-072701]
Ask authors/readers for more resources
Candida rugosa lipase was immobilized on magnetic nanoparticles supported ionic liquids having different cation chain length (C-1, C-4 and C-8) and anions (Cl-, BF4- and PF6-). Magnetic nanoparticles Supported ionic liquids were obtained by covalent bonding of ionic liquids-silane on magnetic silica nanoparticles. The particles are superparamagnetic with diameter of about 55 nm. Large amount of lipase (63.89 mg/(100 mg carrier)) was loaded on the support through ionic adsorption. Activity of the immobilized lipase was examined by the catalysis of esterification between oleic acid and butanol. The activity of bound lipase was 118.3% compared to that of the native lipase. Immobilized lipase maintained 60% of its initial activity even when the temperature was LIP to 80 degrees C. In addition, immobilized lipase retained 60% of its initial activity after 8 repeated batches reaction, while no activity was detected after 6 cycles for the free enzyme. (C) 2008 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available