Journal
JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
Volume 59, Issue 1-3, Pages 168-172Publisher
ELSEVIER
DOI: 10.1016/j.molcatb.2009.02.007
Keywords
Candida antarctica lipase B; Yeast surface display; Whole-cell biocatalyst; Esterification
Funding
- Chinese Programs for High Technology Research and Development (863 Program) [2006AA020203]
- Science and Technology of Guangdong Province [20062050168]
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A recombinant Saccharomyces cerevisiae displaying Candida antarctica lipase B (CALB) on the cell surface was constructed and used as a whole-cell biocatalyst to catalyze the esterification of hexanoic acid and ethanol for the preparation of ethyl hexanoate, a fragrance compound of liquor. Various reaction parameters affecting the esterification catalyzed by CALB-displaying S. cerevisiae whole-cells were investigated. The optimal reaction conditions were reaction temperature 40 degrees C, hexanoic acid concentration 0.2 M, the ratio of hexanoic acid to ethanol 1:1.25 and the amount of cells 60g/l (17 U/g-dry cell). Molecular sieves (3 angstrom) were added to the reaction medium as the water absorbent. The yield reached 98.2% after reaction for 12 h under the optimal conditions. The CALB-displaying S. cerevisiae whole-cell biocatalyst exhibited quite a good operational stability in the esterification, and more than 95% of its original activity was retained after 10 batches reaction. Thus, CALB-displaying S. cerevisiae whole-cell biocatalyst is promising for esters synthesis in non-aqueous phase. (c) 2009 Elsevier B.V. All rights reserved.
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