Journal
JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
Volume 57, Issue 1-4, Pages 164-170Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.molcatb.2008.08.011
Keywords
Alginate; Bitter gourd peroxidase; Concanavalin A; Entrapment; Immobilization; Starch
Funding
- University Grants Commission
- Department of Science and Technology, Government of India, New Delhi
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Calcium alginate-starch hybrid gel was employed as an enzyme carrier both for surface immobilization and entrapment of bitter gourd peroxidase. Entrapped crosslinked concanavalin A-bitter gourd peroxidase retained 52% of the initial activity while surface immobilized and glutaraldehyde crosslinked enzyme showed 63% activity. A comparative stability of both forms of immobilized bitter gourd peroxidase was investigated against pH, temperature and chaotropic agent; like urea, heavy metals, water-miscible organic solvents, detergent and inhibitors. Entrapped peroxidase was significantly more stable as compared to surface immobilized form of enzyme. The pH and temperature-optima for both immobilized preparations were the same as for soluble bitter gourd peroxidase. Entrapped crosslinked concanavalin A-bitter gourd peroxidase showed 75% of the initial activity while the surface immobilized and crosslinked bitter gourd peroxidase retained 69% of the original activity after its seventh repeated use. (C) 2008 Elsevier B.V. All rights reserved.
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