Journal
JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
Volume 56, Issue 4, Pages 215-220Publisher
ELSEVIER
DOI: 10.1016/j.molcatb.2008.05.007
Keywords
DNA-silver nanohybrids; PDDA-protected gold nanoparticles; Hydrogen peroxide biosensor; HRP
Funding
- National Natural Science Foundation of China [20675064]
- Natural Science Foundation of Chongqing City [CSTC-2004BB4149, 200513134100]
- China and High Technology Project Foundation of Southwest University China [XSGX 02]
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A novel amperometric hydrogen peroxide biosensor based on the immobilization of horseradish peroxidase (HRP) on DNA-silver nanohybrids (DNA-Ag) and poly(diallyldimethlammonimum chloride) (PDDA)-protected gold nanoparticles (PDDA-AU) Was Successfully fabricated by combining the self-assembly technique with an in situ electrochemical reduction of the DNA-Ag+ complex. The preparation process of modified electrode was characterized with UV-vis absorption spectroscopy, transmission electron microscopy (TEM) and atomic force microscope (AFM). The electrochemical characteristics of the biosensor were studied by cyclic voltammetry and chronoamperometry. Experimental conditions influencing the biosensor performance such as pH, potential were optimized. The resulting biosensor (HRP/DNA-Ag/PDDA-Au/DNA-Ag/Au electrode) showed a linear response to H2O2 over a concentration range from 7.0 mu m to 7.8 mM with a detection limit of 2.0 mu M (S/N = 3) under optimized conditions. The apparent Michaelis-Menten constant (K-M(app)) was evaluated to be 1.3 mM. The sensor exhibited high sensitivity, good accuracy and an acceptable stability. Crown Copyright (C) 2008 Published by Elsevier B.V. All rights reserved.
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