Journal
JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
Volume 57, Issue 1-4, Pages 177-182Publisher
ELSEVIER
DOI: 10.1016/j.molcatb.2008.08.013
Keywords
Arginine-based surfactants; Enzymatic synthesis; Araujiain; Funastrain; Asclepain c; Hieronymain; Papain; Bromelain
Funding
- ANPCyT [PICT 9-9916]
- CONICET [PIP 2813, 685/04]
- CYTED [IV.22]
- UNLP
- Generalitat de Catalunya DURSI [2005-SGR-00698]
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Partially purified preparations with proteolytic activity, obtained from South American native plants, were used as biocatalysts in condensation reactions of N-protected arginine alkyl ester derivatives with decylamine and dodecylamine in low-water content systems. The final products are cationic surfactants with potential application as emulsifiers and preservatives. Most of the proteolytic extracts were obtained from latex of species belonging to the Asclepiadaceae family (araujiain from Araujia hortorum, asclepain c from Asclepias curassavica and funastrain from Funastrum clausum). Hieronymain was obtained from unripe fruits of Bromelia hieronymi (Bromeliaceae), Plant proteases from commercial sources (papain and bromelain) were also tested as catalysts in the same reactions. Araujiain and funastrain furnished good reaction conversions (60-84%, with a ratio synthesis/hydrolysis of 2-5) similar to those obtained with commercial papain. Moreover, araujiain was the biocatalyst which rendered the best conversions (60%) for the synthesis of the two novel Bz-Arg-NH-dodecylamide (Bz-Arg-NHC12) and Bz-Arg-NH-decylamide (Bz-Arg-NHC10) derivatives. Moderate to poor conversions (10-50%, showing a ratio synthesis/hydrolysis of 0.5-1) were achieved with asclepain c, hieronymain and bromelain. The screening presented in this work revealed that, although these are structurally similar, their behavior for the synthesis of this kind of products differ among them. (C) 2008 Elsevier B.V. All rights reserved.
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