Journal
JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
Volume 54, Issue 1-2, Pages 35-41Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.molcatb.2007.12.005
Keywords
alkyl ferulates; feruloyl esterases; enzyme immobilization; CLEA; transesterification
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Commercial rnulticomponent enzyme preparations, Ultraflo L, Depol 740L and Depot 670L, with feruloyl esterase activity, were tested for the transesterification of methyl ferulate to 1-butyl ferulate in their free and immobilized form using as a reaction system a ternary water-organic mixture consisting of n-hexane, 1-butanol and water. A number of factors affecting enzymes precipitation and cross-linking into cross-linked enzyme aggregates (CLEAs) have been investigated. Consecutive optimization of the precipitant type and cross-linker concentration resulted in CLEAs showing higher operational stability and synthetic activity compared to the free enzymes' forms. Under certain optimization conditions. conversion yields of 97%, 87% and 5%, were obtained by CLEAs prepared from Ultraflo L, Depol 740L and Depol 670L, respectively. The activities initially present in the three commercial preparations were completely retained after cross-linking resulting in multipurpose biocatalysts which have the potential to carry out different and independent reactions. This work is consistent to the novel CLEA concept called combi-CLEA. (C) 2007 Elsevier B.V. All rights reserved.
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