Journal
JOURNAL OF MOLECULAR CATALYSIS A-CHEMICAL
Volume 328, Issue 1-2, Pages 1-7Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.molcata.2010.06.005
Keywords
Cooperative effects; Cyclodextrins; Enzyme models; Host-guest systems; Inclusion compounds
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Funding
- Japan Society for the Promotion of Science
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Three kinds of cyclodextrin (CD) dimer (one homo-dimer of beta-CD and two types of hetero-dimer of alpha-CD and beta-CD) were synthesized as artificial hydrolases. The initial rates of the cleavage reaction of p-nitrophenyl methoxyethoxyethoxyacetate (8) by each of the three dimers were measured under the condition of excess of substrate at 25 degrees C in a pH 7.4 phosphate buffer solution. In a plot of initial rate against concentration of the substrate, the reaction with the homo-dimer (beta C beta H 5) gave a sigmoidal curve, whereas the reaction with either of the hetero-dimers gave a simple hyperbolic curve. Only beta C beta H 5 showed homotropic cooperativity, with a Hill constant of 1.8. (C) 2010 Elsevier B.V. All rights reserved.
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