Journal
JOURNAL OF MOLECULAR BIOLOGY
Volume 426, Issue 15, Pages 2703-2716Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.jmb.2014.05.014
Keywords
bacteriophages; integrases; genome engineering; integrating vectors; synthetic biology
Categories
Funding
- Biotechnology and Biological Science Research council [BB/K003356/1]
- Biotechnology and Biological Sciences Research Council [BB/K003356/1] Funding Source: researchfish
- Engineering and Physical Sciences Research Council [EP/K034359/1, EP/H019154/1] Funding Source: researchfish
- BBSRC [BB/K003356/1] Funding Source: UKRI
- EPSRC [EP/H019154/1, EP/K034359/1] Funding Source: UKRI
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Within the last 25 years, bacteriophage integrases have rapidly risen to prominence as genetic tools for a wide range of applications from basic cloning to genome engineering. Serine integrases such as that from phi C31 and its relatives have found an especially wide range of applications within diverse micro-organisms right through to multi-cellular eukaryotes. Here, we review the mechanisms of the two major families of integrases, the tyrosine and serine integrases, and the advantages and disadvantages of each type as they are applied in genome engineering and synthetic biology. In particular, we focus on the new areas of metabolic pathway construction and optimization, biocomputing, heterologous expression and multiplexed assembly techniques. Integrases are versatile and efficient tools that can be used in conjunction with the various extant molecular biology tools to streamline the synthetic biology production line. (C) 2014 The Authors. Published by Elsevier Ltd.
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