Journal
JOURNAL OF MOLECULAR BIOLOGY
Volume 421, Issue 1, Pages 27-29Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.jmb.2012.05.013
Keywords
NEDD8; ubiquitin; p53; Caspase 7; ubiquitin E1 enzyme
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Funding
- ERC
- European Regional Development Fund Grant for an Innovation Pipeline for Translational Science [LUPS/ERDF/2008/2/1/0429]
- Scottish government
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Ubiquitin and ubiquitin-like proteins use unique E1, E2, and E3 enzymes for conjugation to their substrates. We and others have recently reported that increases in the relative concentration of the ubiquitin-like protein NEDD8 over ubiquitin lead to activation of NEDD8 by the ubiquitin E1 enzyme. We now show that this results in erroneous conjugation of NEDD8 to ubiquitin substrates, such as p53, Caspase 7, and Hif1 alpha, demonstrating that overexpression of NEDD8 is not appropriate for identification of substrates of the NEDD8 pathway. (C) 2012 Elsevier Ltd. All rights reserved.
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