Journal
JOURNAL OF MOLECULAR BIOLOGY
Volume 423, Issue 1, Pages 14-16Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.jmb.2012.06.037
Keywords
defense mechanism; bacteriophage; CRISPR adaptation; DNA template
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Funding
- Israel Science Foundation [611/10]
- Marie Curie International Reintegration Grant [PIRG-GA-2009-256340]
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We analyzed sequences of newly inserted repeats in an Escherichia coli CRISPR (clustered regularly interspaced short palindromic repeats) array in vivo and showed that a base previously thought to belong to the repeat is actually derived from a protospacer. Based on further experimental results, we propose to use the term duplicon for a repeated sequence in a CRISPR array that serves as a template for a new duplicon. Our findings suggest the possibility of redrawing the borders between repeats, spacers, and protospacer adjacent motifs. (c) 2012 Elsevier Ltd. All rights reserved.
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