Journal
JOURNAL OF MOLECULAR BIOLOGY
Volume 412, Issue 5, Pages 814-831Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.jmb.2011.01.024
Keywords
single molecule; transcription; RNA polymerase
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Funding
- NIH [GM074739, GM082848]
- National Science Foundation
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Single-molecule techniques have emerged as powerful tools for deciphering mechanistic details of transcription and have yielded discoveries that would otherwise have been impossible to make through the use of more traditional biochemical and/or biophysical techniques. Here, we provide a brief overview of single-molecule techniques most commonly used for studying RNA polymerase and transcription. We then present specific examples of single-molecule studies that have contributed to our understanding of key mechanistic details for each different stage of the transcription cycle. Finally, we discuss emerging single-molecule approaches and future directions, including efforts to study transcription at the single-molecule level in living cells. (C) 2011 Elsevier Ltd. All rights reserved.
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