Journal
JOURNAL OF MOLECULAR BIOLOGY
Volume 388, Issue 2, Pages 262-270Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.jmb.2009.03.015
Keywords
globin; oxygen sensor; c-di-GMP; diguanylate cyclase; biofilm
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Funding
- National Science Foundation [MCB0446431, 620531]
- U.S. Army Telemedicine and Advanced Technology Research Center [W81XWH0520013]
- National Institutes of Health Intramural Research Program at the National Center for Biotechnology Information
- National Library of Medicine
- National Institutes of Health
- Welch Foundation [I-1575]
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Globin-coupled sensors are heme-bolding signal transducers in Bacteria and Archaea in which an N-terminal globin controls the activity of a variable C-terminal domain. Here, we report that BpeGReg, a globin-coupled diguanylate cyclase from the whooping cough pathogen Bordetella pertussis, synthesizes the second messenger bis-(3'-5')-cyclic diguanosine monophosphate (c-di-GMP) upon oxygen binding. Expression of BpeGReg m Salmonella typhimurium enhances biofilm formation, while knockout of the BpeGReg gene of B. pertussis results in decreased biofilm formation. These results represent the first identification a signal ligand for any diguanylate cyclase and provide definitive experimental evidence that a globin-coupled sensor regulates c-di-GMP synthesis and biofilm formation. We propose that the synthesis of c-di-GMP by globin sensors is a widespread phenomenon in bacteria. (C) 2009 Elsevier Ltd. All rights reserved.
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