Journal
JOURNAL OF MOLECULAR BIOLOGY
Volume 382, Issue 4, Pages 870-883Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.jmb.2008.07.059
Keywords
RNA degradosome; RNase E; DEAD box helicase; polynucleotide; phosphorylase; Hfq
Categories
Funding
- European Union Early-Stage Training Site ChemBioCam
- Wellcome Trust
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The Escherichia coli RNA degradosome is a multienzyme assembly that functions in transcript turnover and maturation of structured RNA precursors. We have developed a procedure to reconstitute the RNA degradosome from recombinant components using modular coexpression vectors. The reconstituted assembly can be purified on a scale that has enabled biochemical and biophysical analyses, and we compare the properties of recombinant and cell-extracted RNA degradosomes. We present evidence that auxiliary protein components can be recruited to the 'superprotomer' core of the assembly through a dynamic equilibrium involving RNA intermediaries. We discuss the implications for the regulation of RNA degradosome function in vivo. (C) 2008 Elsevier Ltd. All rights reserved.
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