Journal
JOURNAL OF MOLECULAR BIOLOGY
Volume 381, Issue 4, Pages 860-866Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.jmb.2008.06.039
Keywords
membrane protein; ClcA; topology; protein structure; fusion protein
Categories
Funding
- Swedish Research Council
- Marianne and Marcus Wallenberg Foundation
- Swedish Foundation for Strategic Research
- Swedish Cancer Foundation
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The topology of bacterial inner membrane proteins is commonly determined using topology reporters such as alkaline phosphatase and green fluorescent protein fused to a series of C-terminally truncated versions of the protein in question. Here, we report a detailed topology mapping of the Escherichia coli inner membrane H+/Cl- exchange transporter ClcA. Since the 3-D structure of ClcA is known, our results provide a critical test of the reporter fusion approach and offer new insights into the ClcA folding pathway. (C) 2008 Elsevier Ltd. All rights reserved.
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