Journal
JOURNAL OF MICROSCOPY
Volume 249, Issue 1, Pages 1-7Publisher
WILEY
DOI: 10.1111/j.1365-2818.2012.03682.x
Keywords
Coherent x-ray diffraction; frozen-hydrated imaging; ptychography; scanning x-ray diffraction microscopy
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Funding
- U.S. Department of Energy, Office of Science [DE-AC02-98CH10886]
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We developed cryo-scanning x-ray diffraction microscopy, utilizing hard x-ray ptychography at cryogenic temperature, for the noninvasive, high-resolution imaging of wet, extended biological samples and report its first frozen-hydrated imaging. Utilizing phase contrast at hard x-rays, cryo-scanning x-ray diffraction microscopy provides the penetration power suitable for thick samples while retaining sensitivity to minute density changes within unstained samples. It is dose-efficient and further minimizes radiation damage by keeping the wet samples at cryogenic temperature. We demonstrate these capabilities in two dimensions by imaging unstained frozen-hydrated budding yeast cells, achieving a spatial resolution of 85 nm with a phase sensitivity of 0.0053 radians. The current work presents the feasibility of cryo-scanning x-ray diffraction microscopy for quantitative, high-resolution imaging of unmodified biological samples extending to tens of micrometres.
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