Journal
JOURNAL OF MICROSCOPY
Volume 249, Issue 1, Pages 8-12Publisher
WILEY
DOI: 10.1111/j.1365-2818.2012.03683.x
Keywords
Cameleon; Ca2+ imaging; FRET; plant mitochondria
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Funding
- Italian Ministero dell'Istruzione, dell'Universita e della Ricerca (MIUR) [RBFR10S1LJ_001]
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Mitochondria are key organelles involved in many aspects of plant physiology and, their ability to generate specific Ca2+ signatures in response to abiotic and biotic stimuli has been reported as one of their roles. The recent identification of the mammalian mitochondrial Ca2+ uniporter opens a new research area in plant biology. To study the mitochondrial Ca2+ handling, it is essential to have a reliable probe. Here we have reported the generation of an Arabidopsis transgenic line expressing thegenetically encodedprobe Cameleon D3 cpv targeted to mitochondria, and compared its properties with the already known Cameleon YC3.6.
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