Journal
JOURNAL OF MICROSCOPY
Volume 242, Issue 3, Pages 221-227Publisher
WILEY-BLACKWELL
DOI: 10.1111/j.1365-2818.2010.03478.x
Keywords
Vitrification; electron microscopy; tomography; image processing
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Cryo-electron tomography allows three-dimensional visualization of frozen-hydrated, vitrified biological material at molecular resolution. Here, we summarize the most important sample preparation methods and technical aspects relevant for cryo-electron tomography, as well as its recent biological applications from isolated macromolecular complexes to entire cells and tissues.
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