Journal
JOURNAL OF MICROSCOPY
Volume 232, Issue 1, Pages 99-105Publisher
WILEY-BLACKWELL
DOI: 10.1111/j.1365-2818.2008.02075.x
Keywords
electro-active polymer (EAP); harmonic excitation light microscopy (HELM); high resolution; structured illumination; wide-field microscopy
Categories
Ask authors/readers for more resources
Combining total internal reflection fluorescence microscopy with structured illumination allows optical wide-field imaging with sub-100-nanometre resolution. We present a novel objective-launch set-up for standing wave illumination that takes advantage of a tunable transmission diffraction grating and transparent phase shifters actuated by electro-active polymers to control the excitation pattern in three dimensions. Image acquisition is completed in less than 1 s. To reconstruct the extended image spectrum, we apply a new apodization function that results in a lateral resolution of 89 nm for green emission wavelength.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available