Journal
JOURNAL OF MICROENCAPSULATION
Volume 29, Issue 3, Pages 234-241Publisher
TAYLOR & FRANCIS LTD
DOI: 10.3109/02652048.2011.646327
Keywords
recombinant human epidermal growth factor; nano-liposome; percutaneous delivery; skin localization
Funding
- Daewoong Pharmaceutical Co., Ltd, Korea
- Korea Research Foundation (KRF)
- Korea government (MEST) [2009-0067835]
- National Research Foundation of Korea [2009-0067835] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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We encapsulated recombinant human epidermal growth factor (rhEGF) into nano-liposomes (NLs) system for topical delivery. The rhEGF-loaded NLs were prepared using a high pressure homogenization method. Morphology and overall particle distribution of NLs were investigated using transmission electron microscopy (TEM) and high resolution microscope (CytoViva (TM)). Particle size, zeta (zeta) potential and encapsulation efficiency were measured and the percutaneous delivery of NLs was evaluated using Franz diffusion cells and immunofluorescence confocal laser scanning microscopy (CLSM). The mean particle size, zeta potential and encapsulation efficiency of the NLs were 155.57 +/- 2.59 nm, -57.92 +/- 4.35mV and 9.00 +/- 0.39%, respectively. TEM and microscopic analysis showed spherical, very even-sized vesicles approximately 150 nm. The skin permeation and localization of rhEGF were enhanced by NLs. CLSM image analysis provided that the NLs enhanced the permeation and localization of rhEGF in rat skin by facilitating entry through pores of skin.
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