Journal
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
Volume 23, Issue 2, Pages 246-250Publisher
KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY
DOI: 10.4014/jmb.1207.07022
Keywords
Loop-mediated isothermal amplification (LAMP); Staphylococcus aureus; femA; rapid detection; clinical samples; food samples
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Funding
- Science Research Fund of Wuhan Institute of Technology [10113201]
- National Science Foundation for Distinguished Young Scholars of China [31000781]
- Open Project Program of Process of Starch and Vegetable Protein Engineering Research Center of the Ministry of Education
- Fundamental Research Funds for the Central University [2012ZB0022]
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In this study, a loop-mediated isothermal amplification (LAMP) method to rapidly detect Staphylococcus aureus strains was developed and evaluated by extensively applying a large number of S. aureus isolates from clinical and food samples. Six primers were specially designed for recognizing eight distinct sequences on the species-specific femA gene of S. aureus. The detection limits were 100 fg DNA/tube and 10(4) CFU/ml. The LAMP assay was applied to 432 S. aureus strains isolated from 118 clinical and 314 food samples. Total detection rates for the LAMP and polymerase chain reaction assays were 98.4% (306/311) and 89.4% (278/311), respectively.
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