Journal
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
Volume 21, Issue 9, Pages 968-971Publisher
KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY
DOI: 10.4014/jmb.1105.05034
Keywords
D-Mannitol; Leuconostoc species; volumetric mannitol productivity; yield mol%
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Funding
- Korea Research Foundation [2009-0085901]
- Korea government (MEST)
- Ministry for Food, Agriculture, Forestry and Fisheries
- National Research Foundation of Korea [2009-0085901] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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Leuconostoc genus, which comprise heterofermentative lactic acid bacteria, reduces fructose to mannitol by recycling intracellular NADH. To evaluate the mannitol productivities of different Leuconostoc species, 5 stock cultures and 4 newly isolated strains were cultivated in MRS and simplified media containing glucose and fructose (1:2 ratio). Among them, L. citreum KACC 91348P, which was isolated from kimchi, showed superior result in cell growth rate, mannitol production rate, and yield in both media. The optimal condition for mannitol production of this strain was pH 6.5 and 30 degrees C. When L. citreum KACC was cultured in simplified medium in a 2 l batch fermenter under optimal conditions, the maximum volumetric productivity was 14.83 g.l(-1)h(-1) and overall yield was 86.6%. This strain is a novel and efficient mannitol producer originated from foods to be used for fermentation of fructose-containing foods.
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