Journal
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
Volume 20, Issue 4, Pages 693-699Publisher
KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY
DOI: 10.4014/jmb.0911.11022
Keywords
Ribonuclease; Russula delica; antiproliferative; purification
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Funding
- National Grants of China [nyhyzx07-008, 2006BADO7A01]
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An antiproliferative ribonuclease with a new N-terminal sequence was purified from fruiting bodies of the edible wild mushroom Rassula delica in this study. This novel ribonuclease was unadsorbed on DEAE-cellulose, but absorbed on SP-Sepharose and Q-Sepharose. It had a molecular mass of 14 kDa, as judged by fast protein liquid chromatography on Superdex 75 and SDS-polyacrylamide gel electrophoresis. Its optimal pH and optimal temperature were pH 5 and 60 degrees C, respectively. The ranking of its activity toward various polyhomoribonucleotides was poly C>poly G>poly A>poly U. It could inhibit proliferation of HepG2 and MCF-7 cancer cells with an IC50 value of 8.6 mu M and 7.2 mu M, respectively. It was devoid of antifungal and HIV-1 reverse transcriptase inhibitory activity.
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