Journal
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
Volume 19, Issue 12, Pages 1576-1581Publisher
KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY
DOI: 10.4014/jmb.0903.03015
Keywords
Achyranthes japonica; 20-hydroxyecdysone; methicillin-resistant Staphylococcus aureus; synergism
Categories
Funding
- Ministry of Education, Science and Technology (MEST)/Korea Science and Engineering Foundation (KOSEF)
- Vestibulocochlear Research Center (VCRC) at Wonkwang University [R13-2002-055-00000-0]
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The emergence of methicillin-resistant Staphylococcus aureus (MRSA) has led to an urgent need for the discovery and development of new antibacterial agents. As part of an ongoing investigation into the antibacterial properties of natural products, 20-hydroxyecdysone (20E), isolated from the roots of Achyranthes japonica Nakai, was found to be active against MRSA, either alone or in combination with ampicillin (AM) or gentamicin (GM), via checkerboard assay. This study investigated the antibacterial activity of 20E, which exhibited poor antibacterial activity (MIC= 250-500 mu g/ml) against MRSA tested. The combined activity of AM or GE plus 20E against MRSA resulted in fractional inhibitory concentractions (FICs) ranging from 4.00 to 0.031 mu g/ml, respectively. Meanwhile, the FIC index ranged from 0.16-4.50, indicating a marked synergistic relationship between AM, GE, and 20E against MRSA. Time-kill assays also showed a remarkable decrease between the combination and the more active compound. Therefore, this study demonstrated that AM, GE, and 20E can act synergistically in inhibiting MRSA in vitro.
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