Journal
JOURNAL OF MICROBIOLOGY
Volume 50, Issue 6, Pages 1024-1028Publisher
MICROBIOLOGICAL SOCIETY KOREA
DOI: 10.1007/s12275-012-2289-9
Keywords
butanol tolerance; butanol yield; Clostridium acetobutylicum; nitrogen ion beam implantation; fermentation
Categories
Funding
- National Natural Science Foundation of China [21176105]
- Program of the Key Laboratory of Industrial Biotechnology, Ministry of Education, China [KLIB-KF201105]
- Fundamental Research Funds for the Central Universities [JUSRP111A24]
- 111 Project [111-2-06]
- Priority Academic Program Development of Jiangsu Higher Education Institutions
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As a promising alternative biofuel, biobutanol can be produced through acetone/butanol/ethanol (ABE) fermentation. Currently, ABE fermentation is still a small-scale industry due to its low production and high input cost. Moreover, butanol toxicity to the Clostridium fermentation host limits the accumulation of butanol in the fermentation broth. The wild-type Clostridium acetobutylicum D64 can only produce about 13 g butanol/L and tolerates less than 2% (v/v) butanol. To improve the tolerance of C. acetobutylicum D64 for enhancing the production of butanol, nitrogen ion beam implantation was employed and finally five mutants with enhanced butanol tolerance were obtained. Among these, the most butanol tolerant mutant C. acetobutylicum NT642 can tolerate above 3% (v/v) butanol while the wide-type strain can only withstand 2% (v/v). In batch fermentation, the production of butanol and ABE yield of C. acetobutylicum NT642 was 15.4 g/L and 22.3 g/L, respectively, which were both higher than those of its parental strain and the other mutants using corn or cassava as substrate. Enhancing butanol tolerance is a great precondition for obtaining a hyper-yield producer. Nitrogen ion beam implantation could be a promising biotechnology to improve butanol tolerance and production of the host strain C. acetobutylicum.
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