Journal
JOURNAL OF MICROBIOLOGY
Volume 47, Issue 4, Pages 506-513Publisher
MICROBIOLOGICAL SOCIETY KOREA
DOI: 10.1007/s12275-009-0014-0
Keywords
Streptomyces chattanoogensis L10; natamycin; pathway-specific regulatory genes; strain improvement; genetic engineering
Categories
Funding
- National Natural Science Foundation of China [30870033]
Ask authors/readers for more resources
A novel Streptomyces strain, 1,10, which is capable of producing natamycin, was isolated from a soil sample collected from Zhejiang province, China. On the basis of phylogenetic analysis of rpoB gene and 16S rDNA sequences, as well as phenotypic comparison, strain L10 (CGMCC 2644) is proposed to be a previously uncharacterized strain of S. chattanoogensis. By screening a cosmid library of strain L10 and primer walking, a partial sequence of scnRI and the entire sequence of scnRII were obtained, which are orthologues to the pathway-specific positive regulator genes of natamycin biosynthesis in S. natalensis. The engineered S. chattanoogensis D1, generated by inserting an additional copy of scnRII into the chromosome of strain L10, increased its natamycin production by 3.3 fold in YSG medium and 4.6 fold in YEME medium without sucrose.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available