Acinetobacter soli sp nov., isolated from forest soil

A non-motile and rod shaped bacterium, designated strain B1(T), seas isolated from forest soil at Mt. Baekwoon, Republic of Korea. Cells were Gram-negative, catalase-positive, and oxidase-negative. The major fatty acids were 9-octadecenoic acid (C-18:1 omega 9c; 42%) and hexadecanoic acid (C-16:0; 25.9%) and summed feature 3 (comprising iso-C-15:0 2-OH and/or C-16:1 omega 7c; 10.0%). The DNA G+C content was 44.1 mol%. A phylogenetic tree based on 16S rRNA gene sequences showed that strain B1(T) formed a lineage within the genus Acinetobacter and was closely related to A. baylyi DSM 14961(T) (98.6% sequence similarity), followed by A. baumannii DSM 30007(T) (97.4%), A. calcoaceticus DSM 300067 (97.0%), and 3 genomic species (96.8 similar to 7.6%). Phenotypic, characteristics, gyrB gene sequence analysis and DNA-DNA relatedness data distinguished strain B1(T) from type strains of A. baylyi, A. baumannii, and A. calcoaceticus. On the basis of the evidence presented in this study, strain B1(T) represents a novel species of the genus Acinetobacter, for which the name Acinetobacter soli sp. nov. is proposed. The type strain is BIT (= KCTC 22184(T)= JCM 15062(T)).