Journal
JOURNAL OF MICROBIOLOGICAL METHODS
Volume 152, Issue -, Pages 69-72Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.mimet.2018.07.013
Keywords
Galleria mellonella; Virulence; Spectrophotometry
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Funding
- French Laboratory of Excellence project TULIP [ANR-10-LABX-41, ANR-11-IDEX-0002-02]
- CNRS-PEPS [SDV-D-2010-1492]
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We combined spectrophotometry and an original statistical approach to infer bacteria virulence, using the lepidoptera Galleria mellonella as a host model. With this method, it is possible to use a microplate reader to automatize data collection on host survival on batches of 96 samples. The method also allows measurement of pathogen multiplication if GFP labelled bacterial strains are used.
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