4.3 Article

Staphylococcus epidermidis biofilm quantification: Effect of different solvents and dyes

Journal

JOURNAL OF MICROBIOLOGICAL METHODS
Volume 101, Issue -, Pages 63-66

Publisher

ELSEVIER SCIENCE BV
DOI: 10.1016/j.mimet.2014.03.016

Keywords

Safranin; Crystal violet; DMSO; Ethanol; Methanol

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Staphylococcus epidermidis biofilm formed in the presence of the solvents DMSO, ethanol or methanol was quantified using safranin or crystal violet staining protocols. We found that biofilm quantification was the most accurate when safranin protocol was applied. Moreover, both DMSO and ethanol stimulated biofilm formation. (C) 2014 Elsevier B.V. All rights reserved.

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