Journal
JOURNAL OF MICROBIOLOGICAL METHODS
Volume 107, Issue -, Pages 84-91Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.mimet.2014.09.006
Keywords
Horizontal gene transfer; Electro-transformation; Magnetic nanoparticles; Magnetic bacterial cell labeling; Magnetic DNA labeling
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Funding
- Region Rhone-Alpes
- French National Research Agency [ANR-08-CESA-013-01]
- LabEx iMUST - Universite de Lyon, Institute for Multiscale Science Technology [ANR-10-LABX-0064, ANR-11-IDEX-0007]
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A culture independent approach was developed for recovering individual bacterial cells out of communities from complex environments including soils and sediments where autofluorescent contaminants hinder the use of fluorescence based techniques. For that purpose fifty nanometer sized streptavidin-coated superparamagnetic nanoparticles were used to chemically bond biotin-functionalized plasmid DNA molecules. We show that micromagnets can efficiently trap magnetically labeled transformed Escherichia coli cells after these bacteria were subjected to electro-transformation by these nanoparticle-labeled plasmids. Among other applications, this method could extend the range of approaches developed to study DNA dissemination among environmental bacteria without requiring cultivability of recombinant strains or expression of heterologous genes in the new hosts. (C) 2014 Elsevier B.V. All rights reserved.
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