Journal
JOURNAL OF MICROBIOLOGICAL METHODS
Volume 95, Issue 3, Pages 353-356Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.mimet.2013.10.001
Keywords
16S rRNA; Diarrhea; Enteric bacterial pathogen; RE digestion
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Funding
- Department of Biotechnology, Government of India [BT/PR15148/GBD/27/339/2011]
- Indian Council of Agricultural Research, New Delhi
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The study describes a rapid approach for detection of common enteric bacterial pathogens, which involves partial amplification of the 16S rRNA gene by PCR using a colony from selective medium followed by restriction enzyme (RE) digestion using the EcoRI, HindIII and SalI enzymes. On the basis of RE digestion analysis different genera namely, Escherichia, Salmonella, Shigella, Vibrio, Campylobacter, Arcobacter, Yesinia and Listeria were differentiated. (C) 2013 Elsevier B.V. All rights reserved.
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