Journal
JOURNAL OF MICROBIOLOGICAL METHODS
Volume 94, Issue 2, Pages 141-143Publisher
ELSEVIER
DOI: 10.1016/j.mimet.2013.06.001
Keywords
Stenotrophomonas maltophilia; Pulsed Field Gel Electrophoresis (PFGE); Time-saving; Cost efficient
Categories
Funding
- Malaysian Government
- Universiti Putra Malaysia [FRGS 5523628]
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We developed a time-saving and cost-efficient Pulsed Field Gel Electrophoresis (PFGE) method for the typing of Stenotrophomonas maltophilia by modifying the conventional procedures. Our modifications related to the cell suspension preparation, lysis of bacterial cells in plugs, washing steps, and consumption of restriction enzyme. Although few rapid PFGE protocols on Gram-negative bacteria are available, the use of comparatively large amounts of costly reagents prompted us to look for other alternative. Hence, by considering the speed, simplicity, and relatively low cost, the modified protocol may be of more practical value than other established protocols in investigating S. maltophilia nosocomial outbreaks. (C) 2013 Elsevier B.V. All rights reserved.
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