High throughput colorimetric assay for rapid urease activity quantification

A novel high throughput colorimetric urease activity assay was compared to the Nessler method. The new method employs phenol red to determine the urease activity. This method reduces significantly sample processing time and allows real-time investigations. This method is rapid, sensitive, easy, cost-effective, and does not use any toxic chemical reagents. (C) 2013 Elsevier B.V. All rights reserved.