Journal
JOURNAL OF MICROBIOLOGICAL METHODS
Volume 95, Issue 2, Pages 304-311Publisher
ELSEVIER
DOI: 10.1016/j.mimet.2013.09.013
Keywords
Immunochromatography; Monoclonal antibody (MAb); Strip test; Vibrio cholerae
Categories
Funding
- Agricultural Research Development Agency (ARDA) Thailand
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A strip test for the detection of Vibrio cholerae 01 was developed using two monoclonal antibodies (MAbs), VC-223 and VC-1226, specific to the lipopolysaccharides of Vibrio cholerae O1 Inaba and Ogawa serovars. The sensitivity of the test was 5 x 10(5) cfu/mL which was similar to that of dot blot test. The detection limit could be improved to 1 cfu/mL of the original bacterial content after pre-incubation of the bacterium in alkaline peptone water (APW) for 12 h. Detection of V. cholerae O1 in various fresh seafood samples such as shriump, blood clam, mussel and oyster could be performed directly with sensitivities ranged from 5 x 10(5) to 10(6) cfu/mL. After pre-enrichment of the shrimp sample in APW, the detection sensitivities increased to 10(2) to 10 CFU/mL of the original bacterial content after incubation for 12 and 24 h. However, the detection sensitivities were also depending on the content of the other bacteria that might inhibit the growth of V. cholerae during pre-enrichment step. The V. cholerae 01 strip test has advantages in speed, and simplicity in not requiring sophisticated equipment or specialized skills and the sample could be directly examined without requirement for sample processing. (C) 2013 Elsevier B.V. All rights reserved.
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