Journal
JOURNAL OF MICROBIOLOGICAL METHODS
Volume 89, Issue 1, Pages 76-78Publisher
ELSEVIER
DOI: 10.1016/j.mimet.2012.01.015
Keywords
Propidium monoazide; qPCR; Membrane filtration; Water quality; Viability
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Funding
- US EPA [MX-96478707-0]
- UWF Office of Research and Sponsored projects
- UWF
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Propidium monoazide (PMA) was used to differentiate live from membrane-compromised bacteria in PCR methods. We have adapted this technique for use on membrane-filtered water samples and determined its efficacy using qPCR. Independent labs at three institutions replicated these findings. (C) 2012 Elsevier B.V. All rights reserved.
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