Journal
JOURNAL OF MICROBIOLOGICAL METHODS
Volume 89, Issue 2, Pages 95-98Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.mimet.2012.02.013
Keywords
Pseudomonas transformation; Plasmid transformation; Yoshida effect
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Funding
- Ministerio de Ciencia e Innovacion, Instituto de Salud Carlos III
- Spanish Network for Research in Infectious Diseases [REIPI RD06/0008]
- FIS-ISCIII [PI10/0150]
- EU [241476]
- European Development Regional Fund A way to achieve Europe ERDF
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Current protocols of recombinant DNA research, including gene cloning and complementation, quantification of gene expression and tagging with reporter proteins, are usually limited by the availability of effective bacteria transformation tools different from Escherichia colt This is particularly relevant with respect to the Pseudomonas species due to their biotechnological and sanitary importance. Here, we describe an optimized and efficient plasmid transference protocol based on the Yoshida effect, a method that relies on DNA uptake mediated by friction forces. The main advantages of this method are: (i) no competent cell preparation is needed, (ii) cells in any physiological state can be used, (iii) the procedure is performed directly on agar plates and (iv) the protocol, which is neither time-consuming nor labor-intensive, offers good efficiency. This approach promises to become the gold standard for day to day genetic manipulation in Pseudomonas. (C) 2012 Elsevier B.V. All rights reserved.
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