Journal
JOURNAL OF MICROBIOLOGICAL METHODS
Volume 90, Issue 3, Pages 192-196Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.mimet.2012.05.004
Keywords
Biofilm; Spectral imaging; Pseudomonas aeruginosa
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Funding
- NASA [NNX09AO60A]
- NIH [P20 RR021905-06]
- National Center for Research Resources [1S10RR019246]
- NASA [111867, NNX09AO60A] Funding Source: Federal RePORTER
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Conventional laser scanning microscopy for multiple fluorescent stains can be a useful tool if the problems of autofluorescence and cross-talk are eliminated. The technique of spectral imaging was employed to unmix five different fluorophores - ranging in emission from 435 to 665 nm - applied to a Pseudomonas aeruginosa biofilm with overlapping spectra and which was not possible using traditional channel mode operation. Using lambda scanning and linear unmixing, the five fluorophores could be distinguished with regions of differentiation apparent. (C) 2012 Elsevier B.V. All rights reserved.
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