Journal
JOURNAL OF MICROBIOLOGICAL METHODS
Volume 84, Issue 3, Pages 486-489Publisher
ELSEVIER
DOI: 10.1016/j.mimet.2011.01.007
Keywords
Bacterial collections; Bacteriophage; Cryopreservation; Phage preservation; Phage storage
Categories
Funding
- Polish Ministry of Science and Higher Education [1/E-35/BWSN/0082/2008]
- European Union within European Regional Development Fund
- Innovative Economy [POIG.01.01.02-00-008/08]
- [PBZ-MNiSW-04/I/2007]
- [N302 020 32/1820]
- [N N301 192439]
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A universal and effective method for long-term storage of bacteriophages has not yet been described. We show that randomly selected tailed phages could be stored inside the infected cells at 80 degrees C without a major loss of phage and host viability. Our results suggest the suitability of this method as a standard for phage preservation. (C) 2011 Elsevier B.V. All rights reserved.
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