Journal
JOURNAL OF MICROBIOLOGICAL METHODS
Volume 85, Issue 2, Pages 164-169Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.mimet.2011.01.027
Keywords
Listeria innocua; Propidium monoazide; Quantitative real-time PCR; Viability
Categories
Funding
- Research Council of Norway (NFR) [186905]
- foundation NORCONSERV
- Western Norway Regional Health Authority, Helse Vest
Ask authors/readers for more resources
The combination of propidium monoazide (PMA) and quantitative real-time PCR (qPCR) significantly overestimated the fraction of viable Listeria innocua as compared to plate counts and confocal fluorescence microscopy. Our data imply that PMA-qPCR must be used with caution as an analytical tool for the differentiation between viable and dead bacteria. (C) 2011 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available
Share Paper
