Journal
JOURNAL OF MICROBIOLOGICAL METHODS
Volume 84, Issue 2, Pages 343-345Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.mimet.2010.12.002
Keywords
Helicase-dependent amplification (HDA); Isothermal; Mycobacterium tuberculosis complex; IS6110
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In this study, we report the development of a helicase-dependent amplification assay for rapid detection of Mycobacterium tuberculosis (MTB). By applying a step-by-step optimization method, the amplification time from an input of 2-copy MTB genomic DNA was reduced from about 60 min to less than 30 min. (C) 2010 Elsevier B.V. All rights reserved.
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