Journal
JOURNAL OF MICROBIOLOGICAL METHODS
Volume 84, Issue 1, Pages 134-136Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.mimet.2010.10.019
Keywords
Wolbachia pipientis; Purification; Genomics; Symbiosis
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Funding
- Foundation for the National Institutes of Health through the Grand Challenges in Global Health Initiative of the Bill & Melinda Gates Foundation
- Australian Research Council
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Most genome sequencing projects using intracellular bacteria face difficulties in obtaining sufficient bacterial DNA free of host contamination. We have developed a simple and rapid protocol to isolate endosymbiont DNA virtually free from fly and mosquito host DNA. We purified DNA from six Wolbachia strains in preparation for genome sequencing using this method, and achieved up to 97% pure Wolbachia sequence, even after using frozen insects. This is a significant improvement for future Wolbachia and other endosymbiont genome projects. (C) 2010 Elsevier B.V. All rights reserved.
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