Journal
JOURNAL OF MICROBIOLOGICAL METHODS
Volume 82, Issue 3, Pages 330-333Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.mimet.2010.06.014
Keywords
MTT reduction; Formazan; E. coli; Viability
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Funding
- Ministry of Science and Technology of China [2009ZX09306-008]
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To reduce the interference in MTT reduction assay, we developed an improved protocol for evaluating the viability of Escherichia coli cells by conducting MTT reduction in 1.5-mL centrifuge tubes and formazan dissolution in test tubes. Our study is helpful in developing protocols for measuring the viability of other gram-negative bacteria. (C) 2010 Elsevier B.V. All rights reserved.
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