Journal
JOURNAL OF MICROBIOLOGICAL METHODS
Volume 80, Issue 3, Pages 274-280Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.mimet.2010.01.011
Keywords
Antibody microarray; Quellung; Serotyping; Streptococcus pneumoniae
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Funding
- Fondo de Investigacion Sanitaria of the Spanish Government of Health [FIS 06/0578]
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The use of antibody-based miniaturized devices for microbiological applications is a field poorly investigated in the era of more developed molecular amplification techniques. A novel antibody microarray for Streptococcus pneumoniae serotyping was developed, by printing nanolitre volumes of pneumococcal serotype-specific antibodies on multi-well slides. This microarray, which showed high specificity when tested against reference and clinical S. pneumoniae isolates, can be applicable as a faster, cost-effective and accurate serotyping technique for pneumococcal epidemiological studies. (C) 2010 Elsevier B.V. All rights reserved.
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