Journal
JOURNAL OF MICROBIOLOGICAL METHODS
Volume 83, Issue 1, Pages 66-68Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.mimet.2010.07.020
Keywords
Spirochete; Selectable marker; Mutagenesis
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Funding
- United States Public Health Service [DE018221, AI079325]
- NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES [R21AI079325] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF DENTAL &CRANIOFACIAL RESEARCH [R01DE018221] Funding Source: NIH RePORTER
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The primary selectable marker for the genetic studies of Treponema denticola is a hybrid gene cassette containing both ermF and ermAM (ermB) genes. ErmB functions in Escherichia coli, while ErmF has been assumed to confer resistance in T. denticola. We demonstrate here that ErmB is sufficient for erythromycin selection in T. denticola and that the native ermB promoter drives ErmB expression. (C) 2010 Elsevier B.V. All rights reserved.
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