Journal
JOURNAL OF MICROBIOLOGICAL METHODS
Volume 82, Issue 1, Pages 98-101Publisher
ELSEVIER
DOI: 10.1016/j.mimet.2010.03.026
Keywords
PCR-DGGE; Idiopathic interstitial pneumonia; Pneumocystis jirovecii; Metagenomic
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Idiopathic interstitial pneumonias are interstitial lung diseases of unknown etiology which prognosis is usually fatal. Microbiota associated to bronchoalveolar lavage from 20 patients with negative bacterial cultures was explored by 16S-rDNA PCR-DGGE, showing a clearly negative relation among the presence of P. jirovecii and bacterial colonization. This is the first report of in vivo antagonistic relation among fungi and bacteria. (C) 2010 Elsevier B.V. All rights reserved.
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