Journal
JOURNAL OF MICROBIOLOGICAL METHODS
Volume 76, Issue 3, Pages 305-306Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.mimet.2008.11.001
Keywords
Aspergillus niger; Fungi; Protoplast formation; Protoplast transformation; Protoplast regeneration; Protoplast storage
Categories
Funding
- Netherlands Technology Foundation (STW) [7453]
Ask authors/readers for more resources
Novozym 234 has been traditionally used to prepare protoplasts for genetic transformation of fungi. Since it is no longer on the market, a new enzyme cocktail was defined to protoplast Aspergillus niger. The cocktail consists of lysing enzymes from Trichoderma harzianum, chitinase from Streptomyces griseus and beta-glucuronidase from Helix pomatia. (C) 2008 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available
Share Paper
